Isolasi dan Identifikasi Bakteri Termofilik dari Sumber Air Panas di Moso Distrik Muara Tami Kota Jayapura Provinsi Papua

https://doi.org/10.31957/jbp.474

Authors

  • Dirk Y.P. Runtuboi Biologi Department of Cenderawasi University
  • Tri Gunaedi Biologi Department of Cenderawasi University
  • Maria Simonapendi Biologi Department of Cenderawasi University
  • Nadya N.L. Pakpahan Biologi Department of Cenderawasi University

Abstract

Thermophilic bacteria are bacteria that are able to survive in environmental conditions with high temperatures. Thermophilic bacteria are one of the important sources of thermostable enzymes that can be isolated from geothermal environments such as hot springs with temperatures ranging from 50–80 oC. Aims of the study to isolate and identify local isolates of thermophilic bacteria from hot springs in Moso Muara Tami District, Papua. The results showed that 7 isolates (A1, A2, A3, A4, B1, B2, and B3) that were isolated and identified based on phenotypic characters were included in the genus Bacillus.  

Key words: Thermophilic, characterization, isolation, identification, phenotypic.

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Author Biographies

Tri Gunaedi, Biologi Department of Cenderawasi University

Biology

Maria Simonapendi, Biologi Department of Cenderawasi University

Biology

Nadya N.L. Pakpahan, Biologi Department of Cenderawasi University

Biology

References

Agustien, A. 2010. Isolasi dan optimasi Bacillus sp penghasil protease alkali dan keratinase termostabil serta aplikasinya. [Disertasi]. Universitas Padjadajaran. Bandung.

Agustini, R. 2006. The utilization of thermophilic protease which life in hot spring Cangar Batu Malang. Indo. J. Chem. 6(2): 205-211.

Bozoglu, C., H. Selin, B. Alayar, M. Karadayi, and M. Gullece. 2015. Isolation and moelcular characterization of thermophilic bacteria with xylanase activity from thermal springs in Erzurum. Jurnal of Life Sciences and Technologies. 3(1): 32-36.

Bozoglu, C., H. Selin, B. Alayar, M. Karadayi, and M. Gullece. 2015. Isolation and molecular characterization of thermophilic bacteria with xylanase activity from thermal springs in Erzurum. Jurnal of Life Sciences and Technologies. 3(1): 32-36.

Cappuccino, J.G. and N. Sherman. 1992. Microbiology a laboratory manual. 3rd Edition. The Benjamin/Cummings Publishing Company, Inc. California, USA.

Domingguez, A., L. Pastrana, M.A.S. Longo, M.L. Rual, and M.A. Sanroman. 2005. Lipolytic enzyme productions by Thermus thermophilus HB27 in a stirred tank bioreactor. Biochem. Eng. J. 26 : 23.

Goldman, E. and L.H. Green. 2009. Practical handbook of microbiology 2th. CRC Press Taylor & Francis Group. pp: 309-326.

Hastuti, W., A. Agustien., and Nurmiati. 2012. Screening and characterization of amylo-thermophylic bacteria from Semurup hot springs, Kerinci, Jambi. Jurnal Biologi Universitas Andalas. 1(2): 150-155.

Krieg, N.R., J.T. Staley, D.R. Brown, B.P. Hedlund, B.J. Paster, N.L. Ward, W. Ludwig and W.B. Whitman. 2010. Bargey manual of systematic bacterology. Second Edition Volume Four. Springer. London.

Kurnia, K. 2008. Menyelamatkan mikroorganisme Indonesia. Kunia Organik Farming & Research. Artikel lepas.

Ladeira, S.A., C. Erica., A.B. Delatorre., J.B. Barbosa, and M.L. Martins. 2015. Cellulase production by thermophilic Bacillus sp. SMIA-2 and its detergent compatibility. Electronik Journal of Biotecnology. 18: 110-115.

Lestari, P. 2000. Eksplorasi enzim termostabil dari mikro termofil. Hayati. 7: 21-25.

Linawati, M.D. 2005. Identifikasi fragmen Gen 16S rRNA pada bakteri termofilik hasil isolasi dari sumber air panas Batu Raden. [Skripsi]. Universitas Diponegoro. Semarang.

Muharni. 2009. Isolasi dan identifikasi bakteri penghasil kitinase dari sumber air panas Danau Ranau Sumatera Selatan. Jurnal Penelitian Sains. 9: 12-15.

Noel, R.K., T. James., Staley., D.R. Brown., P. Brian. Hedlund., B.J. Paster., N.L. Ward, W. Ludwig and W.B. Whitman. 2010. Bergey’s manual of systematic bacteriology. Second Edition. Volume Four. The Bacteroidetes, Spirochaetes, Tenericutes (Mollicutes), Acidobacteria, Fibrobacteres, Fusobacteria, Dictyoglomi, Gemma-timonadetes, Lentisphaerae, Verrucomicrobia, Chlamy-diae, and Planctomycetes. University of Georgia Athens, GA 30602-2605. USA.

Patasik, I., D.Y.P Runtuboi., T. Gunaedi and Y. Ngili. 2015. Identification and characterization of thermophilic Bacillus sp with protease activity at the fragment of 16S rRNA gene of several hot springs in Merauke, Papua-Indonesia. Der Pharma Chemica. 7(7) : 1-10.

Rahmadani, E.P., A. Agustien dan F.A. Febria. 2015. Isolasi dan karakterisasi bakteri amilotermofilik dari sumber air panas Sungan Medang. Jurnal Biologi Universitas Andalas. 4(1): 119-122.

Runtuboi, D., T. Gunaedi, V. Purnamasari., I. Patasik dan N. Uyo. 2014. Indentifikasi Bacillus thermofilik penghasil protease dari beberapa sumber air panas di Merauke Papua. Prosiding Seminar Nasional Biologi di Jayapura, 7-8 Oktober 2014, hal: 1-6.

Scully, S.M and J. Orlygsson. 2015. Recent advances in second generation ethanol production by thermopilic bacteria. Energies. 8: 1-30.

Thieman, W.J., and A.P. Michael. 2013. Introduction to biotech-nology. Pearson. USA.

Tuntun, M dan M. Huda. 2014. Isolasi dan identifikasi bakteri termofilik dari sumber air panas Way Panas Bumi Natar Lampung Selatan. Jurnal Analisis Kesehatan. 3(1): 297-304.

Zhang, H., Z. Zhang, J. Li, and S. Cai. 2007. Effects of Mg2+ on supported bilayer membrane on a glassy carbon electrode during membrane formation. International Journal of Electrochemical Science. 2: 788-796.

Zuridah, H., N. Norazwin, S.M Aisyah, M.N.A. Fakhruzzaman and N.A. Zaenathul. 2011. Identification of lipase producing thermophilic bacteria from Malaysian hot springs. African Journal of Microbiology Research. 5(21): 3569-3573.

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Published

2018-12-24

Issue

Vol. 10 No. 2 (2018)

Section

Research Articles

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