Analisis Variasi Komponen Fotoprotein dan Aldehid Pada Reaksi Bioluminesen Bakteri Luminesen Vibrio ordalii Asal Perairan Laut Papua

https://doi.org/10.31957/jbp.563

Authors

  • Eva Papilaya Jurusan Fisika FMIPA Universitas Cenderawasih, Jayapura–Papua
  • Rita Sinaga Jurusan Kimia, FMIPA Universitas Cenderawasih, Jayapura–Papua

Abstract

Riset pemancaran cahaya yang dilakukan pada bakteri luminesen semakin banyak dipelajari. Hal ini disebabkan bakteri ini sangat mudah untuk dikerjakan di laboratorium, dan mudah diperoleh. Salah satu bakteri yang telah diisolasi dari laut lokal Papua adalah spesies Vibrio ordalii, dan karakteristik biologi dan fisikanya telah dilakukan. Pemancaran cahaya yang dilakukan oleh bakteri ini terjadi melalui reaksi kimia, yang dikenal sebagai reaksi bioluminesen, yang melibatkan tiga komponen fotoprotein, yaitu lusiferase, lusiferin, dan oksigen. Pada riset ini dilakukan variasi terhadap dua komponen, lusiferase dan lusiferin, dan aldehid sebagai komponen pendukung dalam reaksi bioluminesen. Tujuan yang dicapai adalah untuk mengetahui pengaruh variasi kedua komponen terhadap pemancaran cahaya bakteri. Metode yang digunakan adalah metode assay ditionit dan spektrometri dengan menggunakan spektrofotometer uv-vis. Hasil penelitian menunjukkan bahwa aldehid dengan konsentrasi di atas 0,01% tidak meningkatkan luminesen.

Key words: bioluminescent, Vibrio ordalii, fotoprotein, aldehid, lusiferin, lusiferase. 

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Author Biographies

Eva Papilaya, Jurusan Fisika FMIPA Universitas Cenderawasih, Jayapura–Papua

Jurusan Fisika FMIPA Universitas Cenderawasih, Jayapura–Papua

Rita Sinaga, Jurusan Kimia, FMIPA Universitas Cenderawasih, Jayapura–Papua

Jurusan Kimia, FMIPA Universitas Cenderawasih, Jayapura–Papua

References

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Published

2018-10-20

Issue

Vol. 2 No. 2 (2010)

Section

Research Articles

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